Tips and Tricks for Proper Pipetting

Greifensee, United Kingdom (PressExposure) April 18, 2012 -- Pipettes are used frequently in laboratories and results are dependent on their reliable performance. Are you sure your pipette is dispensing the correct amount? While here are regular pipette checkups that can ensure for functioning and accurate pipettes,did you know that your handling also has a great influence on pipette-performance?

Proper pipetting technique directly affects the success and repeatability of your experiments. Below are a few simple things to remember that will greatly improve your pipetting performance.

• Immersion depth

Immersing the tip too far can cause more liquid to be aspirated. Conversely, if the tip is not immersed far enough, air can be aspirated. Both result in inaccurate volume. Correct tip immersion depth can improve accuracy by up to 5%, so use the recommended depths shown here. (>2000 ?l, use 6-10 mm depth)

• Consistent pipette rhythm and speed

Maintainingconsistent pipetting rhythm and speed will produce optimal and more repeatable results. Fast or "jerky" aspiration can cause splashing, aerosols, shaft contamination and loss of sample volume. With consistent rhythm and speed you can achieve up to 5% accuracy improvement.

• Constant temperature

Theideal temperature for pipetting is at 21.5degree C±1 degree C-thesame used for calibration. Avoid drafty or sunlit areas that could cause the large or sudden temperature changes that compromise aspiration accuracy. Pipetting at a constant temperature can improve results by as much as 5%.

• Vertical immersion angle

Keep the immersion angle vertical or within 20 degree of vertical-angles greater than that can produce inaccurate measurements. For micro volume pipettes, keeping the angle close to vertical can improve accuracy by up to 2.%.

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Contact info:

Mettler-Toledo International Inc.

Im Langacher, Zip Code 8606

Greifensee, Switzerland

E-Mail : ePublicRelations@mt.com

Phone : +41-44-944 33 85

Website : http://www.mt.com

About Mettler-Toledo International Inc.

METTLER TOLEDO specializes in the area of precision instruments for professional use. METTLER TOLEDO laboratory instruments are used in research, scientific, drug discovery, and quality control labs, amongst many others in the pharmaceutical, chemical, food and cosmetics industries. METTLER TOLEDO comprehensive industrial solutions cover the various steps in a host of manufacturing processes at many of the same customers that laboratory serves. Solutions range from receiving raw materials through various manufacturing processes, in-line process control and end-of-line packaging control, to logistics and shipping. Increasingly, these solutions are fully integrated into the customer's IT environment, helping automate their workflows. In food retail, our offering for fresh food management ranges from receiving and pre-packaging, to in-store solutions for self-service departments, deli counters, and checkout terminals.

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Press Release Submitted On: April 18, 2012 at 2:24 am
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